Recitation_1

Recitation_1 - Laura Jacox Recitation 1 Who is the person...

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Laura Jacox 1 Recitation 1 Who is the person standing in front of the board? She is: Laura Jacox Email: aura6852@mit.edu Recitation: Wednesday at 11am Concepts for this week: pH / Buffers/ Titration Curve Amino Acids Peptide bonds Protein Purification Protein Structure/Identification Key Points Henderson Hasselbach Equation pH= pKa + log ([Base]/[Acid]) = pKa + log ([proton acceptor]/ [proton donor]) so pH < pKa, species is primarily protonated Amino acid’s α -amino group has a pka of ~9 so at a ph<9 it will be protonated (--NH 3 +) Amino acid’s α -carboxyl group has a pka of ~2 so at a ph<2 it will be protonated (--COOH) Chiral- a carbon has 4 unique groups attached to it Dehydration reaction to make polypeptides: Amino acid + Amino Acid = H 2 0 + dipeptide with a planar peptide bond Peptide bond = Amide bond How have this week’s concepts been tested in the past? (no promises that it will be tested the same this year) -Prof Yaffe usually does not give a generic chemistry question -Instead: he gives a pH and asks for you to draw amino acids with the correct structure and charge based on the pH -asks multiple choice questions -asks you to characterize amino acids (polar, nonpolar, chiral, achiral, charge, chemical reactivity, hydrogen bonding ability) -gives you an amino acid brainteaser of sorts- requires you to know 3 letter and 1 letter abbreviations in addition to thinking Study Suggestions -go through amino acids and characterize by charge according to pH -learn a.a. structure and abbreviations- you will have a.a. structures given to you on the test BUT Prof. Yaffe’s exams are VERY LONG so knowing structures by heart will increase your efficiency -make sure you understand the basics of pH chemistry, peptide bonds, and amino acid characteristics
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Recitation_1 - Laura Jacox Recitation 1 Who is the person...

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