MCB 41 - Lect3

MCB 41 - Lect3 - Lecture 3(FW Cloning of DNA PCR...

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Lecture 3 (FW) January 28, 2009 Cloning of DNA; PCR amplification Reading assignment: Cloning, 240-245; 286-87; 330 PCR, 270-274; 329. Take Home Lesson (s) from Lecture 2: 1. DNA is a double helix of complementary strands held together by A-T and G-C hdrogen bonds. 2. Cellular DNA is located in chromatin ( which become chromosomes during mitosis) in the nucleus, and is faithfully replicated and distributed in the cell cycle and mitosis. Lecture 3. Cloning and PCR . The primary goal: understanding how knowledge of DNA structure allows two powerful new technologies, molecular cloning and PCR. I. Cloning; What is a clone ? A. Webster. 1) genetically identical cells descended from a single common ancestor, or 2) one or more organisms descended asexually from a single common ancestor, or 3) an exact replica. B. Cloning , now used loosely in popular press to mean “making a clone”. The term clone is used to cover DNA molecules, cells, stem cells, or even an individual organism. II. Molecular Clones A. Viruses are clones of DNA (or sometimes RNA) molecules that use cellular machinery for asexual reproduction of multiple copies. 1. A virus-like DNA called a plasmid can “infect “ bacteria. They have very few genes, but sometimes confer interesting properties on the host bacterium, especially drug resistance. B. Viruses and plasmids can be used as carriers (
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This note was uploaded on 02/08/2009 for the course MCB 57739 taught by Professor Wilt during the Spring '09 term at Berkeley.

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MCB 41 - Lect3 - Lecture 3(FW Cloning of DNA PCR...

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