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Unformatted text preview: (1 mM) l/Vo = 2.4, so V max = 0.42 nmoles minl c) (8 points) Determine the k; for polyoxin. In your calculation, use information you obtain from the graph you drew for part a) and show your calculation and any equation(s) you use. Determine l/ K' m (for polyoxin) from xintercept on graph = 0.44 N From this, K'm = 2.3 mM Determine k j from fonnula such as K'm = Km (l + [I]/K j ), i.e. k j = [I]/(K'm/Km)~l So, k i = 3 x 1O6 /(2.3/l.05)1 = 3 x 106 /1.19 = 2.5 ~.M d) (6 points) The substrate for chitin synthase is uridine diphosphate Nacetylglucosamine, whose structure is shown on the right. Which of structures AE below is most likely to be that of polyoxin? Briefly explain your choice. c Polyoxin is a competitive inhibitor, and would therefore be expected to resemble the substrate UDPGlcNAc, which structure C does most closely....
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 Spring '09
 Spees
 Enzyme inhibitor, chitin synthase, polyoxin

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