Photosynthesis Lab Report.pdf

Photosynthesis Lab Report.pdf - Seta Degann 9/23/08 The...

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Seta Degann 9/23/08 The Effectiveness of Different Wavelengths of Light in Reducing Electrons in in Vitro Chloroplasts Introduction The purpose of this experiment was to first observe what wavelength of light chloroplasts would absorb energy the best and then to test this with different colors of light from the visible spectrum. Plants perform photosynthesis in order to harvest energy, which is necessary to carry out their metabolic functions. Photosynthesis occurs in the chloroplasts of the plant cells. It requires water, carbon dioxide, and light energy in order to produce glucose and oxygen (6H 2 O + 6CO 2 = C 6 H 12 O 6 + 6O 2 ). This occurs in two stages: the light and dark reactions. In the light reaction photons of light excite electrons in the chlorophyll pigment, which triggers a reduction reaction. In a reduction reaction electrons are stimulated and jump “uphill” from atom to atom until they reach an electron acceptor, in Vivo this acceptor is NADP+. ATP is also produced in the light reactions. In the dark reactions NADPH (NADP+ plus electrons) and ATP are used to create glucose. (Campbell et al., 181-191). The electron acceptor used in Vitro in this experiment is DCIP+, which has a deep blue color, when it is reduced (it receives electrons) it turns colorless (Bonner et al., C-3). The plant used for this experiment is Spinacia oleraceaI (spinach). Plants are green because they reflect the green wavelength along the visible spectrum and absorb all of the other wavelengths. Some of these wavelengths are absorbed more than others. When light is absorbed more thoroughly there is an increased tendency to donate electrons and therefore create more NADP+ (DCIP+) (Bonner et al., C- 5).
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Materials and Methods For the first and second experiments we needed to extract the chlorophyll pigment. After having removed the big veins from a large spinach leaf, we used a mortar and pestle to grind up the tissue with 5 ml of 100% acetone and sand (to help break it up). We then folded a Kimwipe and filtered the substance through a funnel into a 13 x 100 mm test tube. We added about half the volume of acetone extract in
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This note was uploaded on 02/09/2009 for the course L 113 taught by Professor Hengeveld during the Fall '08 term at Indiana.

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Photosynthesis Lab Report.pdf - Seta Degann 9/23/08 The...

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