MCB102-SPRING2008-PSET3-ANSWERS - MCB102 Metabolism Problem...

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MCB102 / Metabolism Problem Set #3 Spring 2008 These are example problems, which are similar to those you may see on the final exam. QUESTION 1: TRUE/FALSE. Circle the correct answer, but if the answer is FALSE provide a statement that corrects the one given. (i) The mitochondrial PMF can be used to drive transport reactions across the membrane, e.g., ADP/ATP exchange. TRUE FALSE (ii) Ornithine, Arginine, and Aspartate are all amino acids. TRUE FALSE (iii) Pyruvate makes alanine following transamination in the liver in a parallel reaction to that used in the Cori Cycle to recover glucose from lactate. TRUE FALSE (iv) The QH2 produced by NADH oxidation in Complex I is equal in energy to the QH2 produced by FADH2 oxidation via Complex II. Explain why this so and what it means for the final ATP yield for the oxidation of each type of reduced co-factor. TRUE FALSE (v) The heme cofactor in the protein cytochrome c can accept two electrons. TRUE FALSE (vi) The F1 subunit of the F1Fo ATP Synthase is an example of an uncoupler. TRUE FALSE [Sorry, but T/F question iv is a short answer really . My mistake. Anyway, if QH2 is made either from succinate or NADH oxidation it will, of course, have the same redox potential. The production of QH2 via Complex I pumps 4 net protons to the intermembrane space, but the same is not true for oxidation of succinate via Complex II (where no protons are pumped). Thus the 4 proton differential means that 1 less ATP can be produced for succinate oxidation as compared to NADH oxidation.]
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The Urea Cycle occurs in the matrix of mitochondrial so that it may couple by-product fumerate to conserve energy during urea production. T/F TRUE FALSE (vii) The electron transport chain minimizes producing radical intermediate species, like semiquinones, because they cause damage to cellular machinery. TRUE
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This note was uploaded on 02/08/2009 for the course MCB 102 taught by Professor Staff during the Spring '08 term at Berkeley.

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MCB102-SPRING2008-PSET3-ANSWERS - MCB102 Metabolism Problem...

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