Exp#9StandardCurves

Exp#9StandardCurves - Chem 11 Pre-lab Assignment Exp 9...

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Chem 11 Exp. # 9 Standard Curves Pre-lab Assignment 1. Review in Zumdahl, Appendix Three, Spectral Analysis , pages A18-A21. 2. Briefly explain: Beer's Law; The units in Beer's Law; The relationship between absorbance and % transmittance; The independent and dependent axes in a standard curve plot. Introduction Standard curves are often used as tools for determining the concentration of an analyte in an unknown sample. To generate a standard curve, the absorbances of a series of solutions with known concentrations are measured at a specific wavelength (see Figure 1a). The absorbance of the unknown sample is then measured at the same wavelength and compared to the known solutions (see Figure 1b). Beer's law shows the relationship between the concentration and the absorbance of an analyte (see Figure 1c). Figure 1a Figure 1b Figure 1c Common dyes used as food coloring will be analyzed in this experiment. The widely used dyes, Red #40 or Blue #1, will be studied. Solutions of pure Red #40 or pure Blue #1 will be prepared and measured.
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This note was uploaded on 02/10/2009 for the course CHEM 12 taught by Professor Dewald during the Spring '08 term at Tufts.

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Exp#9StandardCurves - Chem 11 Pre-lab Assignment Exp 9...

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