DNA Structure - Lecture 14

DNA Structure - Lecture 14 - . Fig. 14.4 Fig. 14.3 Fig....

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DNA REPLICATION Chapter 14 Raven et al.,
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STUDY OBJECTIVE QUESTIONS #11 DNA Structure Raven et al., Chapter 14 1. What is the experimental evidence that DNA is the hereditary material? 1. What is transformation? Which experiment proved this process? 1. What do DNA and RNA stand for? What are purines and pyrimidines.? 1. Draw a nucleotide and show how it is connected to another with a 3’ to 5’bond. What is the connecting bond? 1. What are Chargaff’s rules? 1. What is meant by semi-conservative replication? Describe the experiment which proved this concept. 1. Describe the process of replication using diagrams. 1. What is the one gene/one polypeptide hypothesis? 1. Draw a model of Watson and Cricks 3 dimensional structure of DNA. Describe this model.
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Fig. 14.1 Transformation - passage of genetic material from one strain to another
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Unformatted text preview: . Fig. 14.4 Fig. 14.3 Fig. 14.2 But what is the genetic material? Therefore DNA is the genetic material. Fig. 14.5 Fig. 14.6 Fig. 14.7 Fig. 14.8 Fig. 14.9 Fig. 14.10 Chargaffs Rules 1.G = C and A = T 2.Composition of DNA varies from one species DNA has the variability to be genetic code COMPLEMENTARITY IS THE KEY. . BUT HOW? Fig. 14.11 Fig. 14.12(top) E. coli grown in 15 N medium Shifted to 14 N and grown Samples taken at 3 time-points Suspended in CsCl solution Spun at high speed Gradient formed DNA found its position on gradient Fig. 14.12(bottom) Therefore DNA replication must be semi-conservative Fig. 14.13 Page 265 Fig. 14.14 Fig. 14.16 Fig. 14.17.a Fig. 14.18 Fig. 14.22 Fig. 14.23 Fig. 14.26 Fig. 14.26 Fig. 14.8 Fig. 14.9 Tab. 14.1...
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DNA Structure - Lecture 14 - . Fig. 14.4 Fig. 14.3 Fig....

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