Gene Function - Lecture 15

Gene Function- - Fig 14.11 Model for Replication Fig 14.12(top E coli grown in 15N medium Shifted to 14N and grown Samples taken at 3 time-points

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Fig. 14.11 Model for Replication?
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Fig. 14.12(top) E. coli grown in 15 N medium Shifted to 14 N and grown Samples taken at 3 time-points Suspended in CsCl solution Spun at high speed Gradient formed DNA found its “position” on gradient Meselson-Stahl
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Fig. 14.12(bottom) Therefore DNA replication must be semi-conservative
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Fig. 14.13
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Page 265 RNA Polymerase does not need a primer DNA polymerases do : Need a primer, Template and Synthesize in a 5’ to 3’ direction DNA Pol I, II and III all 3’ to 5’ exonucleases - proof reading DNA Pol 1 has 5’ to 3’ exonuclease activity
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Fig. 14.14 Prokaryotes Single precise sequence - oriC
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Fig. 14.16 Replication Fork
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Fig. 14.17.a “Sliding Clamp” Subunit of DNA polymerase encircles DNA
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Fig. 14.18 DNA fragments synthesized on the lagging strand are Okazaki fragments DNA primase is an RNA polymerase
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replication of DNA during cell division …. . Replisome
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This note was uploaded on 04/17/2008 for the course HUMANITIES 111 taught by Professor Blume during the Winter '08 term at Union.

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Gene Function- - Fig 14.11 Model for Replication Fig 14.12(top E coli grown in 15N medium Shifted to 14N and grown Samples taken at 3 time-points

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