Enzymes Lab Report - Enzymes Mariah Howes BSC 2010C Section...

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Unformatted text preview: Enzymes Mariah Howes BSC 2010C Section 0025 February 17 th , 2007 By signing below, I guarantee that I am the sole author of this laboratory report. Name: Date: Introduction: The purpose behind this lab is to demonstrate the effect of pH and temperature on enzymatic activity (Walters et al. 2007). Enzymes are considered the most important type of protein. Proteins are made up of amino acids, which is an organic molecule that is made up of both carboxyl and amino groups. Amino acids also serve as the monomer to the protein. Proteins consist of one and sometimes more polypeptides. These polypeptides are folded or coiled in their specific conformation. We get the word protein from the Greek word proteios which means fist place (Campbell and Reese 2005). This is significant because proteins are important in just about everything that organisms do. The higher the level of protein structure, the weaker the bond is that holds them together. The weak bonds that hold the stronger bonds together are Hydrogen Bonds, Hydrophilic Interaction, Van der Waals Interactions, and Electrostatic Interactions. And all of these levels of structures are highly sensitive to the change in temperature (Somero 1978). Back to enzymes, since they are the post important type of protein, they need to regulate metabolism because they are catalysts. A catalyst is a chemical that speeds up the chemical reactions that occur inside the cell but they are not consumed or converted in the reaction. Kinetic properties of enzymes are extremely sensitive to temperature. When the temperature in its surroundings is increased, the velocities of the enzymatic reactions are accelerated. This acceleration doubles the reaction velocity with every 10C increase in the temperature (Somero 1978). What ever functions the enzyme is asked to perform will be continued over and over again. Enzymes are also considered the workhorses because they keep the cell running by carrying out the cycle of life...
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This lab report was uploaded on 04/18/2008 for the course BSC 2010c taught by Professor Janewaterman,pamelathomas during the Spring '07 term at University of Central Florida.

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Enzymes Lab Report - Enzymes Mariah Howes BSC 2010C Section...

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