HC70A quiz 3 - Name Marissa Lara Individuals in Group(if...

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Name: Marissa Lara Individuals in Group (if any): Lora Dyakova, Matthew Spotniz HC70A/SAS70A WINTER 2016 PROFESSORS BOB GOLDBERG & JOHN HARADA QUIZ #3 Due Monday, January 25 @ 5 pm in 4121 Terasaki Building Follow Instructions For Quiz #1 QUESTION ONE – 10,000 points Engineering a tobacco plant expressing a bacterial kanamycin R gene in 1983 by scientists working independently in the United States and Europe, ushered in a new era in crop genetic engineering and promised to bring about a new “green revolution.” Since that time, farmers around the globe have grown over a billion acres of genetically engineered crops, making plant genetic engineering the most rapidly adopted new technology in the history of agriculture. Drs. Marc Van Montagu, Robert Fraley, and Mary-Dell Chilton won the 2013 World Food Prize (Nobel Prize for Agriculture) for “inventing” plant genetic engineering. A. OUTLINE the specific role that each component of the plant engineer’s toolbox performs in generating genetically engineered crop plants. 1. Agrobacterium tumefaciens A soil bacterium that contains a large circular double stranded DNA molecule called a plasmid, which sets off an uncontrolled growth of cells, or a tumor growth, in the plant. Plant specific, and its ability to transfer DNA between itself and plants has made it an important tool for genetic engineering. Provides the machinery and housing for the Ti plasmid 2. Ti Plasmid Tumor inducing plasmid, which produces a tumor called a crown gall. This bacterial plasmid gives biotechnologists an ideal vehicle for transferring desired DNA into the plant cell. The tumor gene is replaced with the gene of interest so it can be replicated within the plant cell. 3. Plant Cell Totipotency & Tissue Culture Plants ability to regenerate from a single cell (totipotency) produces a resulting plant that is a genetic replica or clone of the parent cell and this natural ability of plant cells has made them ideal for genetic research. After new genetic material is introduced into a plant cell, the cell rapidly produces a mature plant and the researcher can see the results of the genetic modification in a relatively short time.
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