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418_assign_5 - Name Exercise 5(30 points due Friday April18...

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Name _________________________________ BioMI 418 Microbial Ecology Exercise 5 (30 points) due Friday April18, 2008 Questions for lecture notes… These questions focus on some things you should consider about community fingerprinting methods. Use your class notes to answer the following: 1) There are many methods used by microbial ecologists to generate a community profile or fingerprint, using PCR products generated from amplification of genes from DNA extracted from an environmental sample. a) Name one fingerprinting method that allows you to recover a band (PCR product), PCR amplify it and determine its sequence. b) Name one community fingerprinting method that does not allow you to recover DNA bands for further analysis. 2) Review your notes on carbon isotopes. a) 14 C What does the number “14” indicate about this isotope (compared to 12 C)? b) What is meant by the term “stable” isotope? Were going to focus on Marine Microbiology over the next few lectures but since we can’t cover it all I
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