418_assign_2 - Name Johnson Zhang Exercise 2 (20 points)...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Name Johnson Zhang BioMI 418 Microbial Ecology Exercise 2 (20 points) due Monday Feb 4, 2008 Part I – Using the NCBI site: Go to the NCBI website: http://www.ncbi.nih.gov/ 1) What does “NCBI” stand for? National Center for Biotechnology Information. At the top of the page select “TaxBrowser”. Search for: Escherichia coli by typing the full name in the box and selecting Go! (don’t just click on the E. coli listing on the page.) Select the highlighted Escherichia coli link and you’ll see a list of isolates. Some are harmless to humans others are pathogens. Our first JAD focuses on the genome of E. coli O157:H7 EDL 933. From this list, you’ll see that there is a second E. coli O157:H7 strain Sakai that has been sequenced. 2) Select the E. coli O157:H7 strain Sakai link. Then on the right side of the page, use the Genome sequences 3 ” link to compare the Sakai and EDL 933 genomes. (Use a similar path to bring up the EDL 933 genome info.) What size is the chromosome of each strain? Sakai strain: 5,498,450 nt; EDL 933: 5,528,445 nt What size are the plasmid(s)? Sakai strain: 92,721 nt (pO157) EDL 933: 92,077 nt 3,306 nt (pOSAKI) 3) What taxonomic rank is “Enterobacteriaceae”? Hint: keep selecting the Enterobacteriaceae link until you find this information. Its taxonomic rank is “Family”. Let’s do a BLAST search! From the Blackboard site, retrieve and open the file called “Sequences.txt”. Select and copy the DNA sequence you have been assigned. Go back to the NCBI home page.
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full DocumentRight Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

This note was uploaded on 04/18/2008 for the course BIO 4180 taught by Professor Angert,e. during the Spring '08 term at Cornell University (Engineering School).

Page1 / 3

418_assign_2 - Name Johnson Zhang Exercise 2 (20 points)...

This preview shows document pages 1 - 2. Sign up to view the full document.

View Full Document Right Arrow Icon
Ask a homework question - tutors are online