HMWK 9 2015 - C211 FALL 2015 Homework 9 Name 10 pts Due...

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C211 FALL 2015 Homework 9 Name ___________________________ 10 pts. Due Thursday, Dec. 3 by 5:00 pm. Show all work. No credit will be given for answers with no work shown. Every question in this assignment is discussed in your textbook . Use the keyword index at the back of the book to find this information. 1. (1 pt) The absorbance of a commercial fruit drink product was 0.415 at a 587 nm. What percent of radiation at 587 nm impinged on the sample is blocked by it? % of light blocked = 2. (1 pts) A colorimetric determination for glucose in blood gave an absorbance of 0.314 for a standard concentration of 85 mg/dL. Assuming the data follows a Beer’s law relationship, what is the concentration of an unknown sample that gave an absorbance of 0.743? C unk = 3. a. (0.5 pt) A 3.50 x 10 6 M solution of the dye, Rose Bengal, had an absorbance of 0.124 at 540 nm. What is the molar absorptivity, , of this dye at this wavelength? Assume a 1-cm cuvette; show units. = b) (0.5 pt) What is the concentration of an unknown solution of Rose Bengal, if it had a transmittance of 81.5 % ? C unk =
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4. (0.8 pt) In a spectroscopic experiment, the photon flux observed at the detector for a blank (solvent, buffer and reagent) is 7.34 x 10 4 photons/cm 2 . When analyte is added, the photon flux at the detector is only 4.19 x 10
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