pp_lab_report_potentials - LABORATORY REPORT Activity...

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LABORATORY REPORT Activity: Action Potentials Name: Quincy Dye Instructor: Date: 03.11.2016 Predictions Exceeding threshold depolarization at the trigger zone______the likelihood of generation of an action potential. increases Action potential amplitude decreases with distance Increasing frequency of stimulation to the trigger zone increases number of action potentials Materials and Methods Experiment 1: Effect of Stimulus Strength on Action Potential Generation Dependent Variable membrane potential Independent Variable stimulus strength (voltage) Controlled Variables frequency of stimulation, type of neuron Experiment 2: Effect of Frequency of Stimulation on Action Potential Generation Dependent Variable membrane potential Independent Variable frequency of stimulation Controlled Variables stimulus strength (voltage), type of neuron 1. Which part of the neuron was stimulated? Axon hillock 2. Where was membrane potential measured? axon 3. What was used to measure membrane potential? Patch clamp Results Table 3: Membrane Potentials at Different Stimulation Voltages, by Location. a. Values of maximal depolarization of membrane potential (mV) at different stimulation voltages, by location. Location Stimulation Voltage 0 V (no stimulation ) 2 V 4 V 6 V 8 V Axon hillock -68.9 -63.6 -56.1 31 31.4 Axon -67.4 -72.9 -64 30.7 -67.4 Laboratory Report/ Quincy Dye/ Action Potentials/ / 03.11.2016/ Page [1] of [4]
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b. Action Potential Generation.
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  • Fall '14
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