Colas Individual Lab Report - Analysis of Phosphate Content...

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Analysis of Phosphate Content in Colas Instructor: Dallas Roe November 16, 2015 Chemistry 1010; Laboratory, Section 003 Mansi Joglekar “My signature indicates that this document represents my own work. Outside of shared data, the information, thoughts and ideas are my own except as indicated in the references. I have submitted an electronic copy through Blackboard to be scanned by TurnItIn.com. In addition, I have not given aid to another student on this assignment.” Discussion of Results
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In this experiment, the main instrument we use is the Spectronic 20, which is a spectrophotometer. A spectrophotometer measures the light intensity of a wavelength before and after it passes through a sample solution in two ways: the percent transmittance, which is the ratio of the light intensity before passing through a sample solution and after passing through multiplied by 100%; and absorbance, which is the log of the transmittance. The analytical wavelength is when the absorbance is at a maximum and the percent transmittance is at a minimum; this is important because the higher the absorbance, the lower the concentration that the analysis can reliably measure and the more accurate our results. We find the analytical wavelength by testing the absorbance and percent transmittance of our sample solution across a range of wavelengths until finding the appropriate wavelength. After we find it, we can test dilutions of our sample and find the various absorbance values to create a calibration curve, a graph showing how the variable, the absorbance, varies with the concentration.
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