Colas Group Lab Report - Analysis of Phosphate Content in...

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Analysis of Phosphate Content in Colas Instructor: Dallas Roe November 16, 2015 Chemistry 1010; Laboratory, Section 003 Mansi Joglekar, Andrew Klapka, Thomas Kenneally Our signatures indicate that this document represents the work completed by our group this semester. Goals
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For this experiment, we were told to figure out the phosphate content in various colas or sodas. To do this we needed to do several things: learn how to correctly use the Spectronic (Spec) 20, a spectrophotometer; determine the relationship between the color of a solution and the wavelength of light absorbed by the solution; devise a way to correlate the amount of light absorbed with the concentration of the compound in the solution; and use that information to determine the phosphate content of three colas – Dr. Pepper, Diet Coke, and Coca-Cola. We also needed to determine the analytical wavelength – specifically, where the percent transmittance was the lowest and the absorbance was the highest – and calibration curve for the phosphate ion solution and use that wavelength to measure the dilutions of the solution. These goals were clearly outlined in our laboratory manual. The experiment was conducted over a total of three weeks: learning about the Spec 20, determining the analytical wavelength and calibration curve for phosphate, and analyzing each of the three colas, respectively. Experimental Design Week One was spent familiarizing ourselves with the Spec 20. We made a total of five cuvettes: three with 10 mL of water and one drop of red, blue, and yellow food coloring, respectively; one with 10 mL of 0.001 M KMnO 4 , which was diluted from 0.004 M KMnO 4
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