LEC03 - Southern Blots Separation of macromolecules using...

Info icon This preview shows pages 1–10. Sign up to view the full content.

View Full Document Right Arrow Icon
Southern Blots
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Separation of macromolecules using electrophoresis through a gel matrix DNA is negatively charged, with a fixed mass/charge ratio: it migrates according to size through either agarose gel or polyacrylamide gel DNA can be cleaved by enzymes known as “restriction enzymes” into fragments A B C cut cut
Image of page 2
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Transfer to a membrane is critical since the agarose or acrylamide gels are very fragile and will fall apart when handled Probes are then visualized using an autoradiogram A B C A B C B
Image of page 4
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Stained gel (EtBr) Autoradiogram Hybrid
Image of page 6
If you do a Southern blot using probe A, how many bands will you see? A. 1 B. 2 C. 3 D. none
Image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Molecular biology techniques derived from Southern blots: Northern blots: RNA separated on gel, probed with DNA probe Western blots: proteins separated on gel, probed with antibodies Colony hybridization: Colonies on a plate probed with DNA probe Theme: separate all macromolecules by size and then probe to see only one of two of the molecules
Image of page 8
How do we go from DNA to protein synthesis?
Image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 10
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern