LEC12 - Three events occur during elongation Aminoacyl tRNA...

Info icon This preview shows pages 1–11. Sign up to view the full content.

View Full Document Right Arrow Icon
Three events occur during elongation Aminoacyl tRNA binds to the A-site. [binding] The growing peptide chain is transferred to the incoming amino acid on the tRNA [peptidyltransfer] The tRNA at the A-site moves to the P-site. [translocation]
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
The aminoacyl-tRNA binds to the A site with assistance of EF-Tu-GTP GTP is hydrolyzed and EF-Tu released only after proper base pairing between tRNA and mRNA
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Peptide bond formation 23S rRNA catalyzes peptide bond formation Ribosome stripped of protein can still catalyze peptide bonds No protein near active site
Image of page 4
Proteins are synthesized from the N-terminus to the C-terminus
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Peptide Bond formation
Image of page 6
Translocation: movement of tRNAs and mRNA EF-G-GTP binds to factor binding site GTP hydrolysis causes conformational change, pushing tRNAs from P and A site to E and P site
Image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
IF-2, EF-Tu and EF-G are all examples of G proteins that undergo large conformational changes upon GTP hydrolysis EF-Tu-GDP EF-Tu-GTP
Image of page 8
Structural mimic
Image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Summary of elongation Binding aa-tRNA with assistance by EF-Tu- GTP GTP hydrolysis Peptide bond formation N on incoming aa-tRNA attacks carbonyl on peptide-tRNA, catalyzed by 23S rRNA
Image of page 10
Image of page 11
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern