LEC09 - Transcription Central Dogma of Molecular Biology...

Info icon This preview shows pages 1–12. Sign up to view the full content.

View Full Document Right Arrow Icon
Transcription
Image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Central Dogma of Molecular Biology Gene expression
Image of page 2
Transcription: synthesis of RNA from a DNA template
Image of page 3

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
How does RNA polymerase (P) know where to start? How does RNA P know which strand to use as template? How does RNA P separate the DNA strands? What is the chemical reaction? What happens if it makes a mistake? How does RNA P know where to stop?
Image of page 4
Overview Enzyme - RNA polymerase Chemical mechanism Steps of transcription Initiation Elongation Termination RNA Polymerases and Transcription Basics
Image of page 5

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
The first RNA polymerase in E.coli DNA Template: ATATATATATAT + rNTP + Mg 2+ + [ 32 P]NTP In vitro reaction Partially purified RNA polymerase Long RNA (incorporated)
Image of page 6
If a template with the sequence GTGTGTGTGT was used which nucleotides would have given radioactive incorporation? A. 32 P-GTP B. 32 P-TTP C. 32 P-UTP D. 32 P-CTP
Image of page 7

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
RNA Polymerase Bacterial polymerase has 5 subunits Closely related to all other RNA polymerases (Archaea, plants and animals)
Image of page 8
Image of page 9

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 10
  and
Image of page 11

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 12
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}

What students are saying

  • Left Quote Icon

    As a current student on this bumpy collegiate pathway, I stumbled upon Course Hero, where I can find study resources for nearly all my courses, get online help from tutors 24/7, and even share my old projects, papers, and lecture notes with other students.

    Student Picture

    Kiran Temple University Fox School of Business ‘17, Course Hero Intern

  • Left Quote Icon

    I cannot even describe how much Course Hero helped me this summer. It’s truly become something I can always rely on and help me. In the end, I was not only able to survive summer classes, but I was able to thrive thanks to Course Hero.

    Student Picture

    Dana University of Pennsylvania ‘17, Course Hero Intern

  • Left Quote Icon

    The ability to access any university’s resources through Course Hero proved invaluable in my case. I was behind on Tulane coursework and actually used UCLA’s materials to help me move forward and get everything together on time.

    Student Picture

    Jill Tulane University ‘16, Course Hero Intern