mIIAw14 - LS3 MIDTERM II EXAM INSTR:L.JOHNSON...

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LS3 MIDTERM II EXAM INSTR: L. JOHNSON             February 28, 2014 NAME:_________________________________ (last) (first) I.D. #__________________________________ TA ___________________________________ Test Form A INSTRUCTIONS This exam is 10 pages long.  Before starting make sure you have all the pages. Using a number 2 pencil, on the front of your scantron fill in the Instructor (Johnson), Class  (LS3), Hour (12:00 or 1:00), Write and Bubble in your UID, your last name and first name. On the back of your scantron Write and Bubble in your UID and Test Form (see above). 1
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1. In vitro translation was used to decipher the genetic code. In the graph above, the bars labeled (-) represent a. the negative control where no 14 C-labeled amino acids were added to the reaction. b. the negative control where no Poly(U) was added to the reaction. c. the negative control at time 0. d. None of the above. 2. In the second step in the tRNA charging reaction, what small molecule is released? 3. In the picture of the ribosome above, what is the order the tRNA binding sites (from left to right)? a. A-P-E b. E-P-A 4. If you plated E. coli on media with glucose + X-gal, what color would the colonies be? a. colorless b. blue 2
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5. Suppose you knocked-out alpha 2 (so that the protein was no longer functional) in a diploid cell. Which sets of genes would be expressed? 6. What do the translation factors, IF-2 and EF-Tu have in common? 7. Which of the following statements is true? a. Rho-independent terminators in prokaryotes are similar to transcription termination in eukaryotes. b. Rho-dependent terminators in prokaryotes and transcription termination in eukaryotes both require special proteins. c. Transcription termination in both prokaryotes and eukaryotes is linked to poly-A addition
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