Review Session - Comparison of gene expression in...

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Comparison of gene expression in prokaryotes and eukaryotes (be able to distinguish and identify differences) Regulatory systems (understanding via what would happen if an aspect is mutated or missing) Components of translation tRNAs 2 Tranestification reactions Charging step involves utilizing energy, raises energy of amino acid so second step is a ‘downhill reaction’ For each amino acid, there is one amino acyl tRNA (20 amino acids, 20 aminoacyl) Wobble pairing – wobble table given, be able to work with it girlfriendddd Ribosomes, polysomes Know size of prokaryotic one Know how large, small subunits are sized rRNA, r-proteins Three sites: why they’re important where they’re located, function etc. Phases of translation Initiation IF-1, IF-2(G protein), IF-3: Know all translation proteins that are G which are G proteins which are not Shine-Delgarno sequence (recognized by small subunit, recognized through base pairing with the 16s rRNA) Formylated Methionine so it looks like a peptide bond (exclusively prokaryotes***) Ternary complex formation – Total ribosome (large and small) initiating tRNA. Ifs have left. Pre-initiation – 30 S subunit on it Initation – 70 S on it (both ternary complex) Final stage of initiation with the 70 S Elongation EF-tu (brings in amino acyl tRNA) ,EF-G (both are G proteins) Binding of aa-tRNA to A site (initiator is in psite) Know chemistry of the peptide bond formation Translocation from one site to the next, facilitated by EF-g (ribosome is moving relative to mRNA and tRNA) shifts A site over so it’s empty tRNA now at p site, but the tRNA is still bonded to the same place on the mRNA Termination RF1,RF2 (depends on what stop codon you use), RF3 (helps release these) RRF(release everybody else) EF-G (helps in translocation) and IF3 play role (helps dissociate the two subunits) Stopcodon is in A site Release factors along with mimic tRNA (confroamtion) Bond between tRNA and polypeptide hydrolyzed (starts termination process)
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Prokaryotic Gene regulation 3 levels of transcription – basal (neither activated nor repressed), repressed,
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  • Winter '12
  • Lin
  • DNA, lac repressor, amino acyl tRNA, eukaryotes vs prokaryotes

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