lec02a - Hershey-Chase Experiment You should be familiar...

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Hershey-Chase Experiment You should be familiar with from MB Radioisotopes Ultracentrifugation
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Elements are defined by the number of protons they possess Electrons = protons in non-ionized forms #Neutrons can vary leading to different isotopes Radioisotopes
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Additional radioisotopes used in Molecular Biology: P 15 32 - important for labeling nucleic acids and phosphorylated proteins S 16 35 - important for labeling proteins mass # atomic #
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Radioactive decay Various particles are emitted (eg.  particles, -rays, etc) Several detection methods are available geiger counters (emissions in air) scintillation counters (radioactive emissions are converted to light) autoradiography (X-ray film) autoradiogram
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Most biological radioisotopes are beta-emitters Equivalent to an electron Vary in energy 3 H- low energy 32 P-high energy Half-life (t 1/2 )- time at which 1/2 of substance has decayed 3 H-12.3 years 32 P-14.3 days
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Ultracentrifuge: Centrifuge that spins at very high speeds allowing separation of bacteria and phage Cells (pellet) Phage (supernatent)
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Hershey-Chase: What happens to the phage DNA and protein upon infection of bacteria?
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