study guide exam 1 BIOL 231

study guide exam 1 BIOL 231 - 5BIOLOGY 231(LABORATORY STUDY...

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5BIOLOGY 231 (LABORATORY) STUDY GUIDE: EXAMINATION 1 1. Study the parts of the microscope and the function of each part. Eye Piece Lens – the lens at the top that you look through . They are usually 10x or 15x power Tube - connects the eyepiece to the objective lenses Arm - supports the tube and connects it to the base Base - The bottom of the microscope , used for support Illuminator – a steady light source Stage – the flat platform where you place your slides . Stage clips hold the slides in place . Moves up , down , left and right. Revolving nosepiece – This is the part that holds two or more objective lenses and can be rotated to easily change Objective Lenses - usually you will find 3 or 4 objective lenses on a microscope . 4x ,10x , 40x , 100x Condenser lens - The purpose of the condenser lens is to focus the light into the specimen 2. Define resolution. What is the resolution of a compound microscope with oil immersion lenses? What happens to working distance and resolution at higher magnifications? What are the factors that contribute to maximum resolution? Resolution – the smallest distance between two points on a specimen that can still be distinguished as two separate entities The highest resolution on a compound microscope is 100x It Becomes smaller Higher resolution to the object . The closer you are to the object the wider the possible cone of light 3. Why is it important to have blue filter over the light source? - Because the shorter wave length of the resulting light will provide maximum resolution 4. At what position should the condenser be kept in order to get maximum resolution? - The condenser should be kept at the highest position that allows the maximum amount of light to enter objective lens and therefore limit the amount of light lost due to refraction 5. What is the use of immersion oil when observing slides under the microscope?
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- When placed between the specimen and objective lens , the oil forms a continuous lens system that limits the loss of light due to refraction 6. List the various solvents that are used for cleaning the microscope lenses.
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