EXPERIMENT 10CARBOHYDRATESName:Dhara PatelDebbie Lo (Lab Partner)Date:April 11, 2008Objective:To compare and contrast different carbohydrates reactions of monosaccharide, disaccharides and polysaccharides by observing melting point of oszone of glucose and fructose solution and to acknowledge how enzymatic hydrolysis of a glycosidic linkage occurs. Also, to understand how reducing and non-reducing sugar visually by using Benedict’s reagent for glucose, fructose, lactose, sucrose, starch, sucrose with yeast and starch with yeast. Discussion:In this lab, there were three different parts by studying glucose osazone, fructose osazone by using phenylhydrazine reagent, understanding enzymatic hydrolysis of a glycosidic linkage, and observing reducing sugars by Benedict’s test. For the first part, it clearly showed that glucose took much long time than fructose to form osazone. Both test tube containing glucose and fructose in each was started at 12:50 pm.
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