Ruhi Patel molec cell mini lab.docx - Ruhi Patel What...

Ruhi Patel
What happens when we increase the concentration of our solution and the optical density
we get. After diluting to different concentrations, our solution was analyzed at 625 nm. Optical
density was directly affected by an increase in concentration. The optical density and
concentration are our y and x, respectively, in terms of the Lambert Beer Law, with the extinction
coefficient serving as our slope.
Concentrati
on Optical Density at 625 nm
0.625 0.019
2.25 0.02
2.5 0.052
5 0.103
7.5 0.168
10 0.233
12.5 0.244
Table 1. Fast green Standard Curve Data.
Using a Fast Green sample diluted in seven cuvettes with increasing dilution measures at 625
nm, the standard curve shown in Figure 1 was created.
Figure 1. Standard curve for Serial Dilutions of the Fast Green Solution
The scatter plot of the seven cuvettes from Table.1 with a Lambert Beer Law-indicated
best fit line shows the results of the experiment. The figure shows that the optical density
measured at 625 nm increases as concentration increases.