ANA CHEM - LIQUID CHROMATOGRAPHY GROUP MEMBERS SHARWIN...

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LIQUID CHROMATOGRAPHY
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GROUP MEMBERS SHARWIN KUMAR A/L SAMBETH KA15128 GOH EVE KA15220 NUR SYAZA HAMNII BINTI MOHD ADNAN KA15232
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PRINCIPLES AND THEORY
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Liquid chromatography (LC) is an analytical chromatographic technique that is useful for separating ions or molecules that are dissolved in a solvent.
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Chromatography is used to separate proteins, nucleic acids, or small molecules in complex mixtures. Liquid chromatography (LC) separates molecules in a liquid mobile phase using a solid stationary phase. Liquid chromatography can be used for analytical or preparative applications
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All chromatographic systems have a mobile phase that transports the analytes through the column and a stationary phase coated onto the column or on the resin beads in the column. The stationary phase loosely interacts with each analyte based on its chemical structure, resulting in the separation of each analyte as a function of time spent in the separation column .
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The less analytes interact with the stationary phase, the faster they are transported through the system. The reverse is true for less mobile analytes that have stronger interactions. Thus, the many analytes in a sample are identified by retention time in the system for a given set of conditions
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STATIONARY PHASE: Phase that stay inside the column and it can be a solid, gel or highly viscous liquid MOBILE PHASE: (a liquid or gas) It is the solvent moving through the column
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Technique for separation of mixture component (either for analytical / preparative purpose) Sample being analyse in liquid / gas form Involves physical Technique of measurement based on measurement spectra Sample being studied is ionized Involves heating a chemical compound in DIFFERENCES BETWEEN CHROMATOGRAPHY AND SPECTROSCOPIC CHROMATOGRAPHY SPECTROSCOPIC
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TYPES/VARIATION AND DESCRIPTION OF INSTRUMENTS
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REVERSE PHASE NORMAL PHASE TYPES OF CHROMATOGRAPHY
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In normal phase chromatography, the stationary phase is polar , and so the more polar solutes being separated will adhere more to the stationary adsorbent phase.
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