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Unformatted text preview: We blanked the Spec using the distilled water solution by setting it to 100%. Then finally we took down the transmittance level numbers. 5) • 1.0% BSA darkest in color when tested-more blue • Milk solution lightest when tested • 5.0% BSA was more purple • Distilled water had the highest transmittance value of 98 while the 5.0% BSA had the lowest with a 22 • We took down the transmittance values down instead of the absorbency values 6) See graph 7) We wrote down the transmittance measurements instead of the absorbency measurements. We don’t have any averages, only one set of numbers derived from the spectrophotometer. 8) From this activity, I learned to pay more attention to directions and not make careless mistakes like taking down the wrong data. I could have been more attentive and read the lab better....
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- Fall '07
- Proteins, Laboratory glassware, Pipette, Pasteur pipette, bulbs-10 ml pipettes