Enzm kin-2 3-7-16 - Enzymes Kinetics and Inhibition Lecture...

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Lecture – 2 3-7-2016 Enzymes: Kinetics and Inhibition
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Enzyme Inhibition Studies of enzyme inhibition are very valuable because they give information on the active site of an enzyme, its mechanism of action and possible mechanisms of regulation. This is of physiological significance
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Inhibitors The activity of many enzymes can be inhibited by binding specific small molecules and ions An irreversible inhibitor dissociates very slowly from its target enzyme because it has become very tightly bound to the enzyme (either covalently or non-covalently) Reversible inhibition is characterized by a rapid dissociation of the enzyme-inhibitor complex A competitive inhibitor binds to the active site and prevents the substrate from binding An uncompetitive inhibitor binds only to the enzyme- substrate complex A non-competitive inhibitor does not prevent the substrate from binding
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Enzymes can be Inhibited by Specific Molecules
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Competitive Inhibition Key properties: The enzyme can bind EITHER S or I but not both simultaneously.
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  • Spring '16
  • Pande,Jayanti
  • Enzyme, pH, Kinetics, Enzyme inhibitor, inhibition, enzymesubstrate complex, VMAX S VMAX

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