10 NEW TRANSCRIPTION - PCB 3023 Lecture Outlines...

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PCB 3023 Lecture Outlines TRANSCRIPTION GOALS OF LECTURE Enzymes Gene Structure Concepts of Transcription RNA Processing Genetic Code ORDER OF FIGURES 7-6 7-15 7-3 7-19 7-5 7-20 7-8 7-9 7-10 7-13 7-14 Key terms to know A, C, G, U mRNA, tRNA, rRNA, snRNA Promoter Coding Region Terminator RNA Polymerase Start Site Terminator 5’ 3’ Intron Exon Spliceosome Alternative Splicing Transcription Factors
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PCB 3023 Lecture Outlines LECTURE OUTLINES TRANSCRIPTION RNA POLYEMRASE High energy triphosphates SIMILARITIES BETWEEN DNA REPLICATION AND TRANSCRIPTION Carried out by series of enzymes including a polymerase Involves production of linear polymer of nucleotides connected by phosphodiester bonds • Synthesis is 5’->3’ Uses 4 distinct nucleotides Adenine (A) Uracil (U) Cytosine (C) Guanine (G) DIFFERENCES Nucleotides used are ribonucleotides Uracil instead of thymine RNA is SINGLE STRANDED
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Lecture Outlines Strands of RNA can self pair •RNA itself can function as an enzyme TYPES OF RNA Messenger RNA (mRNA) Ribosomal RNA (rRNA) Transfer RNA (tRNA) Small RNA (snRNAs) Each RNA is synthesized by a distinct RNA Polymerase QUESTION? How does the RNA polymerase know where to start and stop transcription of a specific gene and know which direction to go?
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This note was uploaded on 04/19/2008 for the course PCB 3023 taught by Professor Sikorski during the Fall '07 term at University of South Florida - Tampa.

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10 NEW TRANSCRIPTION - PCB 3023 Lecture Outlines...

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