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Unformatted text preview: Lab Exercises #3 and #4: Molecular Biology I and I I 1) E.Coli is the name of the bacteria that will grow on the plates. 2) A plasmid is not a living cell; rather it is DNA that is NOT chromosomal. I t is circular and double stranded. The plasmid can glow, and it is different from the genomic DNA because they are separate. Also, the plasmid is not like the genomic DNA because it is not connected to the bacterial cell wall through proteins. 3) The LB agar plates are called a phenotypic identification because if there is no growth on the plate where there is LB agar, than there must be something else there that is not allowing the bacteria to grow. LB is Luria Broth which promotes bacterial growth. 4) If E.Coli has the plasmid pAMP, then it would be resistant to ampicillin. pAMP could grow on LB, LB-AMP, and LB-AMP/ARA. There would be no growth on the LB-KAN plate. 5) If E.Coli has the plasmid pKAN, then it would be resistant to kanamycin. pKAN could grow on LB and LB-KAN. There would be no growth on the LB- AMP or LB-AMP/ARA plate. 6) If E.Coli has the plasmid pGLO, then it would be resistant to ampicillin. pGLO could grow on LB, LB-AMP, and LB-AMP/ARA. It would glow on the LB-AMP/ARA plate. There would be no growth on the LB-KAN plate....
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This note was uploaded on 04/19/2008 for the course CHEM 0120 taught by Professor Golde during the Spring '07 term at Pittsburgh.
- Spring '07