05 Transformation

05 Transformation - Recombinant DNA technology Challenges:...

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1 Transformation and Biotech Products Recombinant DNA technology Challenges: 1. isolate target gene recombinant DNA construct 3. put vector into “factory” cell 4. select cells with plasmid allow them to replicate 5. convince cells to produce protein 6. harvest product 13.7 Figure 13.7 Figure 13.7 figure 13-07.jpg Prokaryotes: Reproduction, Mutation, and Recombination • A bacterium can transfer its genes to another bacterium by conjugation, transformation, or transduction.
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2 13.10 figure 13-10a.jpg Figure 13.10 Figure 13.10 – Part 1 Part 1 13.10 figure 13-10b.jpg Figure 13.10 Figure 13.10 – Part 2 Part 2 Transformation: Most common in recombinant DNA projects This is still a bit of an art, but several methods have been developed that induce a few bacteria to take up DNA. • Use a plasmid: many bacterial plasmids are naturally exchanged between organisms.
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05 Transformation - Recombinant DNA technology Challenges:...

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