BIO320_2016_I_DNA2protein_to post - Genetics part 2 from genotype to phenotype Maurice Kernan Dept of Neurobiology Ctr for Developmental Genetics office

BIO320_2016_I_DNA2protein_to post - Genetics part 2 from...

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Genetics, part 2: from genotype to phenotype Research: I use Drosophila to study the mechanical senses (touch, hearing, coordination). office: Centers for Molecular Medicine, room 447 (just over the 4th floor bridge from Life Sciences) Hours: Tu/Th 4-5pm, Weds 2-3pm, or email for an appointment: [email protected] Maurice Kernan Dept. of Neurobiology & Ctr. for Developmental Genetics Right now, I am especially interested in a regulated alternative splice that may differentiate phasic vs. tonic mechanosensory neurons
Genetics, part 2: from genotype to phenotype There will be clicker questions Now posted on Blackboard: • Lecture Powerpoints. Files posted in advance may differ from version shown: - some illustrations omitted (to save trees) - some clicker questions included Echo recordings Study Guides • Question banks (keys posted separately) QB I, sections A-E covers ~lectures # 15-17. • New Discussion forum
Lectures 14-15+: Mechanisms of gene expression understand how the genetic code was solved experimentally, how it is read in cells, and its implications for evolution and the origin of life on Earth (QB sections A, B). Study Guide I Question bank sections A-G be able to describe mechanisms and follow the information flow from DNA to protein in transcription and translation, including RNA splicing and processing in eukaryotes (QB sections D, E, G). be able to predict the RNA and protein products of given DNA sequences, and the effects of specific mutations on the structure and function of encoded proteins (QB sections B, C, F, G). Learning objectives for this section :
Proteins carry out most biochemical functions Genes control phenotype because they encode proteins. DNA is biochemically inert DNA Protein Code: “a system of symbols that equates information in one language with information in another.” (Hartwell, ch 8.1) Genotype Phenotype
Genotypebase sequenceDNAbase sequenceRNAPhenotypeamino acid sequenceProteinInformationflows from DNA to RNA to protein - the “Central Dogma” of molecular biology.1. How the genetic code was solved experimentally2. How the code is “read” in cells
Amino acids & polypeptide structurePhenylalanineTyrosineArginine
The “sequence hypothesis”: all properties of a protein are determined by its primary structure: the amino-acid sequence.

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