Methods with px~(2008)

Methods with px~(2008) - Biology 304 Histology S Michele...

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Biology 304 - Histology S. Michele Robichaux, D.D.S. Histology – Methods I. Introduction A. Definition 1. Organology- study of organ system 2. Histology – study of tissues 3. Cytology – study of cells B. Relationship to other sciences (Gross Anatomy, Physiology, Morphology) II. Microscopy (page 12-22) A. Units of measurements 1. Micrometer (10 -6 ) μ m 2. Nanometer (10 -9 ) nm 3. Angstrom (10 -10 ) Å B. Microscopy terminology 1. Resoloution 2. Magnification 3. Numerical aperture: related to the width of the lens aperture (the greater the NA, the greater the resolving power) 4. Optical components of a microscope: a. Condenser b. Objective c. Ocular C. Light Microscopy – 1. Features of the light microscope a. Light source, glass lens b. Magnification c. Refractive index (1) Immersion oil 1
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2. Types of light microscopes a. Compound Bright Field Microscopy b. Phase Contrast Microscopy c. Other specialized light microscopes: interference microscope, dark field microscope, fluorescence microscope, confocal scanning microscope, ultraviolet microscope, polarizing microscope Interference Microscopy Phase Contrast Microscopy Compound Bright Field Microscopy 2
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