genetics - different recessive mutations yields a mutant...

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5’ overhangs are called sticky ends HindIII ligase creates 5’ overhangs Blunt ends are hard to reanneal 2’ OH in RNA allows it to be catalytic phosphate backbone is on periphery Complementation In genetics, complementation refers to a relationship between two different strains of an organism which both have homozygous recessive mutations that produce the same phenotype (for example, a change in wing structure in flies). These strains are true-breeding for their mutation — when crossed with themselves they will always produce offspring with the mutant phenotype. If, when these strains are crossed with each other, some offspring show recovery of the wild-type phenotype, these strains show "genetic complementation". - * If the combination of two haploid genomes containing
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Unformatted text preview: different recessive mutations yields a mutant phenotype, then the mutations must be in the same gene. If the combination of two haploid genomes containing different recessive mutations yields the wild type phenotype, then the mutations must be in different genes. * the functional definition of a gene is the complementation test + virus can immediately be used to make protein-virus must first copy themselves before making mRNA host range phenomenon- some viruses have harder time attacking cells leading to ugly plaques b/c less cells are infected to determine if a phage is lysogenic, infect a previously infected cell, if they are resistant, then the phage is lysogenic turbid plaques are lysogenic cells in the center of a viral plaque...
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This note was uploaded on 04/20/2008 for the course BIO 350 taught by Professor P.gauss during the Spring '08 term at Western State Colorado University .

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