BIO 206L: Exercise 2 Analysis
1. What is Köhler illumination and why is important?
2. How does the “field of view” change with magnification? Relate this to the rule that one should alwaysuse the low-power scanning objective first to locate a specimen. How does the “depth of focus” change as magnification increases? Are thick (e.g., Elodea) or thin (e.g., prepared Paramecium) samples of specimens preferred at high magnifications?
3. How does one improve resolution? What does a numerical aperture (N.A.) indicate? What is the relationship between resolving power and numerical aperture?
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- Spring '08
- Unknown
- Biology, Bone marrow, numerical aperture, reticle units
