Lab Analysis 2 - BIO 206L Exercise 2 Analysis 1 What is Khler illumination and why is important Kohler illumination is a technique in light microscopy

Lab Analysis 2 - BIO 206L Exercise 2 Analysis 1 What is...

This preview shows page 1 - 2 out of 5 pages.

BIO 206L: Exercise 2 Analysis 1. What is Köhler illumination and why is important? 2. How does the “field of view” change with magnification? Relate this to the rule that one should alwaysuse the low-power scanning objective first to locate a specimen. How does the “depth of focus” change as magnification increases? Are thick (e.g., Elodea) or thin (e.g., prepared Paramecium) samples of specimens preferred at high magnifications? 3. How does one improve resolution? What does a numerical aperture (N.A.) indicate? What is the relationship between resolving power and numerical aperture?
Image of page 1
Image of page 2

You've reached the end of your free preview.

Want to read all 5 pages?

  • Spring '08
  • Unknown
  • Biology, Bone marrow, numerical aperture, reticle units

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture

  • Left Quote Icon

    Student Picture