Lab Analysis 5 - BIO 206L: Exercise 5 Lab Analysis 1. What...

BIO 206L: Exercise 5 Lab Analysis
1. What is pGLO? Describe the structure of pGLO. Be sure to discuss the function/role of any genes or regions
important for its role as a genetic vector. Give an example of how a plasmid like pGLO can be used by
researchers.
2. Define, in general, what a restriction enzyme is and what it does. Give an example of a restriction enzyme
not discussed in lecture and specifically describe its activity (e.g., restriction site).
3. State the function/role of each of the components in the pGLO + microcentrifuge tube. What happened to the
pGLO plasmid during its time in the incubator?