labreport - The Transforming Genetics Introduction: Genetic...

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The Transforming Genetics Introduction: Genetic transformation is the ability to move DNA into an organism and alter its genotype or genetic make up. In single celled organisms transformation is a procedure that involves e-coli and a stimulus. There are also multi-celled organisms where the transformation is usually more difficult. This is more difficult because it brings in the complications of transforming each cell in the plant instead of just one cell, like in the single celled organisms. The actual transformation of these cells takes place when a cell takes up and expresses a foreign piece of DNA. Methods and Materials: For this experiment we obtained two microcentrifuge tubes and put 250 μ l of calcium chloride into each tube labeled +pGLO and –pGLO. After this we placed both of these tubes into ice bath. Once the tubes were on ice we took a sterile loop and got a single colony of bacteria and put one in each of our tubes. Once the sterile loop is in the solution you want to spin the loop gentle so that the single colony gets off
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This note was uploaded on 04/20/2008 for the course HDFS 217 302 32 taught by Professor All during the Spring '08 term at Colorado State.

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labreport - The Transforming Genetics Introduction: Genetic...

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