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Unformatted text preview: reaction. Use the picture as the beginning (ES complex) state and draw the tetrahedral intermediate and product states. Be sure to indicate movement of electrons with arrows and all formal charges. BMB100B Winter 2009 Rubin 4) Explain why most enzyme active sites are buried within the interior of proteins as opposed to on the protein surface. Try to give three reasons. 5) Aldolase catalyzes cleavage of fructose 1,6-bisphosphate into dihydroxyacetone phosphate and glyceraldehydes 3-phosphate ( read from right to left below ). Aldolase has a catalytic lysine that acts as a nucleophile (think Schiff base intermediate) and residues in the active site that can act as acid/base proton acceptors and donors. Write down a mechanism describing the reaction that contains intermediates, movement of electrons and protons, etc....
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- Spring '09