photosynthesis lab - Abstract In this lab we used paper...

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Abstract In this lab, we used paper chromatography to separate spinach plant pigments and then calculated the R f values of the pigments. Plant pigments function as light absorbers in photosynthesis and transfers light energy to the reaction centers of photosystems. We measured the rate of photosynthesis; boiled chloroplasts vs. unboiled chloroplasts and light vs. dark. The rate of photosynthesis is quicker with the unboiled chloroplasts in the light. We conducted a dye-reduction technique, using DPIP, to measure the rate of photosynthesis. As a result of it being reduced, its color changed from blue to colorless. We used a spectrophotometer to measure the changes of light transmittance over a period of time. Introduction Photosynthesis is the process by which plants, some bacteria, and some protists use the energy from sunlight to produce sugar. Photosynthesis is separated into two stages: light reactions and the Calvin cycle. 6H 2 O + 6CO 2 ----------> C 6 H 12 O 6 + 6O 2 is the equation for photosynthesis. The main objectives of this lab were to separate pigments, calculate their R f values, and study the rate of photosynthesis with isolated chloroplasts. The separation of plant pigments allowed us to identify the different types of pigments and compare our R f values to the standard R f values. Pigments are chemical compounds which reflect only certain wavelengths of visible light. This makes them appear colorful. More important than their reflection of light is the ability of pigments to absorb certain wavelengths. Because they interact with light to absorb only certain wavelengths, pigments are useful to plants and other autotrophs-organisms which make their own food using photosynthesis. In plants, algae, and cyanobacteria, pigments are the means by which the energy of sunlight is captured for photosynthesis. However, since each pigment reacts with only a narrow range of the spectrum, there is usually a need to produce several kinds of pigments, each of a different color, to capture more of the sun's
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photosynthesis lab - Abstract In this lab we used paper...

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