Lab 2 Chemical Separation

Lab 2 Chemical Separation - Chemical Separation Lab 2...

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Chemical Separation Lab 2 Author: Holly Polk Lab Partners: Jim Hernandez Instructor: Yisheng Xu Chemistry Lab 151, Section 001 Date Work Performed: February 4, 2008 Date Report Submitted: February 11, 2008 Abstract: In this experiment, the different pigments are extracted from spinach leaves by grinding, then using the property of solubility to extract color pigments. Many chemical separations are based on the differences in the strength of attractive forces between their particles. The process of this experiment includes extracting pigments from spinach leaves, performing TLC on the spinach leaf extract, and determining the best solvent mixture to use to separate the pigments in the extract.
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Introduction: The experimental objectives of this experiment are to extract pigments from spinach leaves, perform TLC on the spinach leaf extract, and determine the best solvent mixture to use to separate the pigments in the extract. A good solvent for separating colored pigments in spinach leaves is a mixture of acetone and hexane. The main objective of the experiment is to test different samples of prepared mixtures of acetone and hexane to find the combination that work best. The experimental procedure begins by extracting pigments from the spinach leaves. The pigments are located deep in the plant’s leaves, so the cell walls have to be crushed to get them out. So, the leaves must be ground with sand and a solvent in a mortar. After grinding and breaking down the cell walls, the dark green organic layer must be decanted into a small beaker. Then the solvent volume is set aside to evaporate and reduce to about 1 mL. After pigment extraction, thin layer chromatography is performed. First, six
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This note was uploaded on 02/17/2009 for the course CHEM 151 taught by Professor Staff during the Spring '08 term at University of Arizona- Tucson.

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Lab 2 Chemical Separation - Chemical Separation Lab 2...

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