Lab 12 Mushrooms in Chocolate - Lab 12 Mushrooms in...

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Lab 12: Mushrooms in Chocolate?NAME: Jeresa WatsonBefore coming to lab: Review Sections 22.4-22.5 and read the manuscript entitled “Psychedelic Mushrooms in Chocolate: Introduction to GC-FID” found at the end of this document. Do the prelab exercise below.Learning Outcomes: 1.Students will apply chromatography theory to solve the efficiency in a chromatographic separation. Students will explain resolution, theoretical plates, and the van Deemter equation and demonstrate the use of these while analyzing sample data.2.Students will read a manuscript to extract details of an experiment.Prelab Exercise (each is 5 points)1.In your review of Sections 22.4 and 22.5, there are a number of equations. Write out all numbered equations here.
2.Provide the answer and show how you solved it for “test yourself” on page 549.
3.Provide the answer and show how you solved it for “test yourself” on page 553.
4.Provide the answer and show how you solved it for “test yourself” on page 555.
The following is to be done during the lab period. Write the questions in your lab notebook and answer them in your lab notebook.Questions are weighted 10 pts each. Question 1. Using the areas and concentrations of sponge extractions in Table 1 below, define the average response factor F.
Figure 2.Representative GC/FID chromatogram of an extracted, spiked sponge sample.
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Table 1. Concentrations and peak areas of analyte and internal standard of sponge extractions.Conc, 4-AminoacetophenoneConc, TryptaminePeak area, 4-AminoacetophenonePeak area, TryptamineTrial 112.08mg/mL11.95mg/Ml9141568508Trial 212.08mg/mL11.95mg/Ml229061199312Question 2. Using a ruler and pencil, mark Figure 2 and calculate the resloution defined by Resolution = Δtr/Wav. What does this tell us about the separation?
Question 3.Figure 3 shows a representative GC/FID analysis of a chocolate extraction. Using the response factor (question 1), Equation 1, and Table 2 below, calculate the average concentration tryptamine in the chocolate extraction.
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Question 4.If 11.95 mg of Tryptamine was spiked into the chocolate, determine the percent recovery from the extraction. What does this tell you about the method performance?

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