150-15replication[1] - Foundations of Biological Science...

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Foundations of Biological Science I (BioSci 150) C Replication (DNA Synthesis) catalyzed semiconservative bi-directional
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Foundations of Biological Science I (BioSci 150) C Dehydration 0 A Reminder
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Foundations of Biological Science I (BioSci 150) C Hypothesis: The enzyme that catalyzes DNA synthesis should have all of the properties necessary to bind repliacte a complementary double helical DNA molecule. What are some of these properties? Prediction: cells have a DNA polymerase that makes new DNA that looks like parental template DNA. 0
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Foundations of Biological Science I (BioSci 150) C Testing the DNA polymerase hypothesis bacterial culture extract cell proteins Purify DNA from a different organism Conclude: Cell proteins contain a t emplate-dependent DNA polymerase * DNA is made; base ratios ( A:G:C:T) of the new DNA are the same as that of the DNA source. Mix, add 4 * dNTPs, wait… * * * * * * * *
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Foundations of Biological Science I (BioSci 150) C In proposing the complementary double- helical structure of DNA, Watson & Crick had predicted that the strands separate and serve as templates for synthesis of a complementary strand: Iss this the only possibility?
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Foundations of Biological Science I (BioSci 150) C Models of Replication Conservative parental dsDNA is conserved Dispersive strand breakage & repair leads to mosaic strands Check out how Meselson & Stahl used non-radioactive isotopes of N to prove that replication is semi-conservative ... Semi-Conservative “new” DNA = one parental/one new strand
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Foundations of Biological Science I (BioSci 150) C Electron microscopy and Autoradiography
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