Lecture 2 - Eukaryotic Transcription Factors

Lecture 2 - Eukaryotic Transcription Factors - Eukaryotic...

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Eukaryotic Transcription Factors Prokaryotic: Transcription regulated by nutritional and physical conditions (environment) - allosteric regulation of TFs by metabolites (lactose) - repression of transcription Eukaryotes: Increased complexity signalled by increase in transcriptional factors - activation of transcription (versus repression) - cooperation between several specific transcription factors mRNA genes transcribed at low levels unless stimulated by specific TFs e.g. transcription of SV40 virus genes depends on host SP1 (first specific transcription factor isolated from human cells) Viruses hijack cellular transcriptional machinery to transcribe their genes Transcription factor binding sites can be found through linker scanning mutations: replacing small regions of the target DNA at a time, and then testing for functionality – those who are no longer functional have been interrupted Reporter genes facilitate the relative quantification of transcriptional efficacy - Green Fluorescent Protein (GFP), β -galactosidase ( lacZ ), thymidine kinase ( tk ), luciferase (luc), chloramphenicol acetyltransferase (CAT)
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This note was uploaded on 04/22/2008 for the course BIOL 200 taught by Professor Bureau during the Fall '06 term at McGill.

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Lecture 2 - Eukaryotic Transcription Factors - Eukaryotic...

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