{[ promptMessage ]}

Bookmark it

{[ promptMessage ]}

Lecture 2 - Eukaryotic Transcription Factors

Lecture 2 - Eukaryotic Transcription Factors - Eukaryotic...

Info iconThis preview shows pages 1–2. Sign up to view the full content.

View Full Document Right Arrow Icon
Eukaryotic Transcription Factors Prokaryotic: Transcription regulated by nutritional and physical conditions (environment) - allosteric regulation of TFs by metabolites (lactose) - repression of transcription Eukaryotes: Increased complexity signalled by increase in transcriptional factors - activation of transcription (versus repression) - cooperation between several specific transcription factors mRNA genes transcribed at low levels unless stimulated by specific TFs e.g. transcription of SV40 virus genes depends on host SP1 (first specific transcription factor isolated from human cells) Viruses hijack cellular transcriptional machinery to transcribe their genes Transcription factor binding sites can be found through linker scanning mutations: replacing small regions of the target DNA at a time, and then testing for functionality – those who are no longer functional have been interrupted Reporter genes facilitate the relative quantification of transcriptional efficacy - Green Fluorescent Protein (GFP), β -galactosidase ( lacZ ), thymidine kinase ( tk ), luciferase (luc), chloramphenicol acetyltransferase (CAT)
Background image of page 1

Info iconThis preview has intentionally blurred sections. Sign up to view the full version.

View Full Document Right Arrow Icon
Image of page 2
This is the end of the preview. Sign up to access the rest of the document.

{[ snackBarMessage ]}