Lecture 8 - Translational and post-translational

Lecture 8 - Translational and post-translational -...

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Unformatted text preview: Translational and post-translational regulations of gene-expression PABPI binds to both poly-A tail and initiation complex, forms ring, favours reinitiation- some polypeptides under strict regulatory control- protein synthesis is often in proportion to protein abundance more; mRNA = more protein- If this relationship is skewed, then protein synthesis is being mediated by some other mechanism o Either preventing translation or after translation * comparison performed using Western Blot (amount of protein) against Northern Blot (amount of RNA) Ferritin is a protein that binds iron ions, and prevents accumulation of free ions- low iron, IRE-BP is active and binds to IRE on mRNA does not stabilize like in Transferrin- IRE-BP blocks the translation of Ferritin and Fe isnt not bound free Fe in cell should rise Many oocyte mRNAs arent expressed until fertilization- in xenopus, switch is mediated by changes in the poly A tail length- Efficient translation is blocked since PABP1 cannot bind...
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This note was uploaded on 04/22/2008 for the course BIOL 200 taught by Professor Bureau during the Fall '06 term at McGill.

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Lecture 8 - Translational and post-translational -...

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