Lecture 5 - Post-Transcriptional Regulation

Lecture 5 - Post-Transcriptional Regulation -...

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Post-transcriptional regulation of mRNAs Binding of U1A protein to two sequence motifs (U1A binding sites) in its own mRNA does not prevent cleavage of the RNA by the poly(A) signal-specific endonuclease (CPSF), however it prevents addition of the poly(A) tail - RNA fragments are degraded by ribonucleases - U1A protein a part of the U1snRNP - This auto-regulates the amount of U1snRNA active in a cell in a feedback loop - IF there is a lot, the free U1A binds to its own mRNA and it is degraded without poly-A tail (U1A will not be in snRNP) - Ensures that the amounts of U1snRNA and U1A are equilibrated Alternative splicing in fibronectin results in proteins with different properties - two mRNA regions EIIIB and EIIIA encode surface receptor interaction domains - with both, fibronectin is fibroblast specific, surface adherent fibronectin - with neither, hepatocyte-specific, and is serum traveling Alternative splicing also determines sex in Drosophila - early in embryogenesis sxl (sex lethal) gene isoform is translated (two exons) iun
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This note was uploaded on 04/22/2008 for the course BIOL 200 taught by Professor Bureau during the Fall '06 term at McGill.

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Lecture 5 - Post-Transcriptional Regulation -...

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