This preview shows page 1. Sign up to view the full content.
Unformatted text preview: Brown (dominant) babies can be X-/X+ (one X from ES germ line, one from black mouse) or X+/X+: germ line missed Screen for heterozygotes mate heterozygotes screen progeny for homozygotes X-/X- (knockout mice) Transgenic mice (simpler and can provide important information)-transgenic genes injected integrate randomly, integrated into pronuclei (so all of the genes, including germ line, will be altered)-transgenic reporter genes are important for understanding expression patterns-genes can be turned on at specific times in development and can be tissue specific (tissue-specific promoter) to deduce whether gene has activity in certain cell/tissue/organism...
View Full Document
- Fall '06
- molecular biology