Lecture 13 - Proteomics

Lecture 13 - Proteomics - Proteomics- the vast majority of...

Info iconThis preview shows page 1. Sign up to view the full content.

View Full Document Right Arrow Icon
This is the end of the preview. Sign up to access the rest of the document.

Unformatted text preview: Proteomics- the vast majority of identified cells still have an unclear function overexpression of a gene gives information, but also artefacts best method is interruption gene removal by forward genetic analysis, reverse genetic analysis (RNAi) disruption construct: sequence homology to the two flanking regions of target (20-40bp) 2D-electrophoresis 1. separation by charge (pH) isoelectric focussing 2. first gel added to top of second 3. separation by size SDS electrophoresis Mass spectrometry and protein identification- protein digested by trypsin proteolysis- undergoes Liquid chromatography, or more commonly, mass spec- spectral data correspond to peptide mass fingerprint, and will be unique to polypeptide- a genome database analysis will indicate what protein it is Transcription factors are modular: can be separated into DNA binding domain and the Transcriptional activating domain Yeast two hybrid method If protein A is bound to a DNA binding domain and protein B is linked to an activation domain, it can be...
View Full Document

Ask a homework question - tutors are online