Chapter11ProblemSet - Karmelo Navalta(kan337 BIO325(51420...

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Karmelo Navalta (kan337) BIO325 (51420) January 28, 2008 Chapter 11 Problem Set C9) The two types of sequences within bacterial origins of replication that are functionally important are the DnaA box and the A-T rich region. The DnaA box is a binding site for the protein DnaA which then bind to each other and in doing so put pressure on the helix to push strands apart from each other. The A-T rich region works with the DnaA box to help push the strands apart from each other by having a smaller number of hydrogen bonds to separate and also being positioned at exactly where DnaA protein has effect to force it apart. The DnaA box and A-T rich region work together to separate the strands to create an origin and one acting without the other is not enough to change the shape of the strand and push it apart. C15A) The exonuclease activity occurs in the 5’ to 3’ direction during the removal of the RNA primers and in the 3’ to 5’ direction during the proofreading and removal of mistakes following DNA replication. C16) A) The Okazaki fragment made first would be the right Okazaki fragment. B) The primer first removed would be the right Okazaki fragment. It is necessary
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