BIO325 - Lecture 5 - BIO325 January 29, 2008 DNA...

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BIO325 January 29, 2008 DNA Transcription Cont. I. DNA Transcription (cont.) a. Overview and Definitions i. Elongation (cont.) 1. Not both strands used as template, only one a. Other DNA strand is sense strand ii. Template Strand 1. ssDNA used as a template during transcription 2. e.g. 5’-AUGC-3’/3’-TACG-5’ 3. Dependent on gene in question iii. Sense Strand 1. ssDNA strand not used as a template 2. Useful for looking at RNA structure with same polarity and same sequence (switch T and U) 3. Same polarity as RNA 4. Dependent on gene in question iv. Transcription Bubble 1. Stays same size during process, only most recently formed DNA is still bound to template 2. Rest of DNA is trailing as a tail 3. Moves along strand, but stays same size v. Termination 1. RNA pol releases RNA and dissociates from DNA vi. Downstream v. Upstream 1. RNA pol can only go in one direction when bound 2. Downstream a. Direction of RNA pol movement along DNA strand during transcription b. “Flow of Transcription” c. Can pick any two spots and speak relatively of each other i. “Promoter is upstream of gene, Transcription Termination Site is 550 b.p.
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This note was uploaded on 04/22/2008 for the course BIO 325 taught by Professor Saxena during the Spring '08 term at University of Texas at Austin.

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BIO325 - Lecture 5 - BIO325 January 29, 2008 DNA...

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