BIO325 - Lecture 2 - BIO325 1/17/08 DNA Structure and...

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BIO325 – 1/17/08 DNA Structure and Replication A. DNA a. A polymer, chain made of nucleotides b. Nucleotide i. 1-3 Phosphate Groups/ nucleotide ii. One Sugar 1. Deoxyribose 2. Ribose 3. The only truly reliable between DNA and RNA 4. “Defining difference between DNA and RNA” iii. One Base 1. DNA a. Adenine b. Guanine c. Thymine d. Cytosine 2. RNA a. Adenine b. Guanine c. Cytosine d. Uracil 3. Difference between Purines and Pyrimidines a. Purines – A, G (Double Ring) b. Pyrimidines – T,C,U (Single Ring) c. Phosphates attach to 5’ Carbon d. Bases attach to 1’ Carbon B. Naming Nucleotides a. ‘d’ = deoxyribose sugar (Find 2’ Sugar) i. Ribose sugar doesn’t have a distinction b. ‘A’ = adenosine (adenine) c. ‘DP’ = Di-phosphate (two phosphate groups) d. e.g. i. “ATP” 1. Sugar – Ribose 2. Base – Adenosine 3. Phosphate Groups – 3 (Tri-phosphate) C. Linkages in Structure a. Covalent Phosphodiester linkage between bases i. 5’ to 3’ Carbons D. Chargaff’s Rule a. A = T, G = C
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E. Rosalind Franklin
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BIO325 - Lecture 2 - BIO325 1/17/08 DNA Structure and...

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